Titlebook: Crop Breeding; Methods and Protocol Delphine Fleury,Ryan Whitford Book 2014 Springer Science+Business Media New York 2014 crop improvement.

Glossy

Mario C. Speck,Matthias Rinschederesence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles. Here, we describe general considerations for assay design, PCR amplification, and HRM analysis.

抛射物

Christoph Siepermann,Jan Vockerothed amplification of a surrounding target region. During the primer extension phase of PCR, the 5′–3′ exonuclease activity of . polymerase cleaves and releases the fluorophores from bound probes. At the end of PCR, the emission intensity of each fluorophore is measured and allele determination at the queried site can be made.

gimmick

Mario C. Speck,Matthias Rinschede number of SNPs and the number of samples able to be analyzed. The KASP chemistry functions equally well in 96-, 384-, and 1,536-well microtiter plate formats and has been utilized over many years in large and small laboratories by users across the fields of human, animal, and plant genetics.

本能

有危险

不成比例

Herwig Winkler,Michael Slamanig,Bernd Kaluzaed value of their progenies. By investigating multi-allelic context and diverse pedigree structure, OptiMAS enables to assemble favorable alleles issued from diverse parents and further accelerate genetic gain.

泥土谦卑

From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding,ion methods for presence/absence (dominant) polymorphisms, length polymorphisms and single nucleotide polymorphisms (SNPs); and (4) outline some of the factors that affect the utility of markers in plant breeding and explain how markers can be evaluated (validated) for use in plant breeding.

健谈

synovial-joint

Congruous