Titlebook: Craniofacial Development; Methods and Protocol Sebastian Dworkin Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive

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Methodology for Free-Floating Organ Culture of Mid-gestation Maxillary Primordial Tissue,nd multifactorial nature of their etiology. In vitro modeling of craniofacial morphogenesis provides valuable insight into the developmental processes critical to the presentation of these conditions. One such technique, termed a submerged or free-floating organ culture, allows culturing and observa

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Single Cell RNA-Seq: Cell Isolation and Data Analysis, which tissue architecture can be characterized. Multiple cell capturing methods have been developed that, when coupled to next-generation sequencing, can yield cell-to-cell specific information regarding gene expression profiles. One of the commonalities between all of the cell capturing techniques

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,Engineering Epithelial–Mesenchymal Microtissues to Study Cell–Cell Interactions in Development,ining operationally simple and scalable. As an example, formation of the upper lip and palate requires the orchestrated proliferation and fusion of embryonic facial growth centers and is dependent on paracrine epithelial–mesenchymal signaling through multiple pathways including the Sonic Hedgehog (S

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Ex Vivo Culture of Human Cranial Suture Cells, the molecular biology ex vivo. The use of cell cultures by bone biologists to investigate pathological bone formation has been well established (Marie et al. Vitro Cell Dev Biol 25:373–380, 1989), and the use of cell culture techniques was subsequently applied to investigate craniosynostosis (Marie