书目名称 | Craniofacial Development | 副标题 | Methods and Protocol | 编辑 | Sebastian Dworkin | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (.c. elegans., zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and .in vivo. cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using .in-situ .hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. .Authoritative and cutting-edge, .Craniofacial Development: Methods and Protocols. aims to be a guide in the field of craniofacial development for senior and new researchers l | 出版日期 | Book 2022 | 关键词 | Drosophila; zebrafish; scRNA-SEQ; Caenorhabditis elegans; palatal development | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-1847-9 | isbn_softcover | 978-1-0716-1849-3 | isbn_ebook | 978-1-0716-1847-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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