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Titlebook: Craniofacial Development; Methods and Protocol Sebastian Dworkin Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive

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发表于 2025-3-21 17:04:35 | 显示全部楼层 |阅读模式
书目名称Craniofacial Development
副标题Methods and Protocol
编辑Sebastian Dworkin
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Craniofacial Development; Methods and Protocol Sebastian Dworkin Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive
描述.This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (.c. elegans., zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and .in vivo. cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using .in-situ .hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. .Authoritative and cutting-edge, .Craniofacial Development: Methods and Protocols. aims to be a guide in the field of craniofacial development for senior and new researchers l
出版日期Book 2022
关键词Drosophila; zebrafish; scRNA-SEQ; Caenorhabditis elegans; palatal development
版次1
doihttps://doi.org/10.1007/978-1-0716-1847-9
isbn_softcover978-1-0716-1849-3
isbn_ebook978-1-0716-1847-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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发表于 2025-3-21 22:58:16 | 显示全部楼层
Book 2022oaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (.c. elegans., zebrafish, mouse, alongside human imaging approaches), together with cell cultu
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Wavelets in höheren Dimensionenangeable between various vertebrate species, and between various surface imaging techniques (including micro-CT and 3D surface scanners), offering a high level of versatility and precision for extensive phenotyping of the entire craniofacial complex.
发表于 2025-3-22 14:00:49 | 显示全部楼层
Methodology for Free-Floating Organ Culture of Mid-gestation Maxillary Primordial Tissue,ate culturing tissue from developmental time-points as early as embryonic day 10.5. This allows for greater control over genotypic variance within litters and provides a simplified, accessible methodology.
发表于 2025-3-22 20:16:53 | 显示全部楼层
Single Cell RNA-Seq: Cell Isolation and Data Analysis, present a streamlined protocol to isolate fresh cells from tissues in vivo. We also share extensive notes to highlight considerations researchers should take into account before beginning their cell isolation protocol.
发表于 2025-3-22 22:36:18 | 显示全部楼层
Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofaciangeable between various vertebrate species, and between various surface imaging techniques (including micro-CT and 3D surface scanners), offering a high level of versatility and precision for extensive phenotyping of the entire craniofacial complex.
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