Titlebook: Connexin Hemichannels; Methods and Protocol Fabio Mammano,Mauricio Retamal Book 2024 The Editor(s) (if applicable) and The Author(s), under

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,Enhanced Methodologies for Investigating the Electrophysiological Characteristics of Endogenous Panpotential distribution across cellular networks. The family of Pannexin (Panx) proteins, in particular Pannexin1 (Panx1), are widely expressed in vertebrate cells and exhibit sequence homology with innexins, the invertebrate gap junction channel constituents. Despite being ubiquitously expressed, de

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A Protocol for the Automated Assessment of Cutaneous Pathology in a Mouse Model of Hemichannel Dysfhyperactive connexin 26 hemichannels. Reflectance imaging is a versatile and powerful tool in dermatology, offering noninvasive, high-resolution insights into skin pathology, which is essential for both clinical practice and research. This approach offers several advantages and applications. Unlike

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Measuring Connexin Hemichannel Opening in Response to an InsP3-Mediated Cytosolic Ca2+ Increase,ncluding extracellular and intracellular Ca. ions. Submicromolar variations of the cytosolic Ca. concentration ([Ca.].) are per se sufficient to trigger extracellular bursts of messenger molecules through connexin HCs, thus mediating paracrine signaling. In this chapter, we present a quantitative me

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1064-3745 ation advice from the experts.This volume explores the latest developments in the study of connexin hemichannels (HCs). The chapters in this book cover topics such as purification, reconstitution, and functional analysis of connexin HCs; spatial and temporal localization of connexins in cells using

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Mordechai Feingold,Victor Navarro-Brotonsd cause deafness. Studies using purified isolated systems under well-controlled conditions are essential for a full understanding of molecular mechanisms of hemichannel function under normal conditions and in disease, and here, we present methodology for the expression, purification, and functional analysis of hemichannels formed by Cx26.