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Titlebook: Connexin Hemichannels; Methods and Protocol Fabio Mammano,Mauricio Retamal Book 2024 The Editor(s) (if applicable) and The Author(s), under

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书目名称Connexin Hemichannels
副标题Methods and Protocol
编辑Fabio Mammano,Mauricio Retamal
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Connexin Hemichannels; Methods and Protocol Fabio Mammano,Mauricio Retamal Book 2024 The Editor(s) (if applicable) and The Author(s), under
描述.This volume explores the latest developments in the study of connexin hemichannels (HCs). The chapters in this book cover topics such as purification, reconstitution, and functional analysis of connexin HCs; spatial and temporal localization of connexins in cells using confocal microscopy; evaluation of connexin HC activity in vivo; generation of connexin-expressing stable cell pools; and automated assessment of cutaneous pathology in a mouse model of HC dysfunction. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Connexin Hemichannels: Methods and Protocols. is a valuable resource that discusses the importance of HCs as drug targets, and how studying them is essential to identifying newcompounds capable of modulating HC function selectively and specifically.. . .
出版日期Book 2024
关键词membrane proteins; bacteria; exosomes; cellular physiology; ATP release
版次1
doihttps://doi.org/10.1007/978-1-0716-3842-2
isbn_softcover978-1-0716-3844-6
isbn_ebook978-1-0716-3842-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Expression and Function of Connexins in Extracellular Vesicles,y explored for diagnosis and therapeutic purposes. The presence of multiple connexin (Cx) proteins has been described in EVs, where they might facilitate EV–cell communication. However, quantitative changes in Cx levels and functional assessment of Cx channels have only been established for Cx43. In
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Expression, Purification, and Nanodisc Reconstitution of Connexin-43 Hemichannels for Structural Chterface of two neighboring cells. The HCs may be involved in ion and small-molecule transport across the cellular plasma membrane in response to various stimuli. Despite their importance, relatively few structures of connexin HCs are available to date, compared to the structures of the GJCs. Here, w
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Molecular Dynamics Simulation of Permeation Through Connexin Channels, level. They offer possibilities that are often unavailable to experimental methods, making MD an ideal complementary technique for the understanding a plethora of biological processes. Thanks to significant efforts by many groups of developers around the world, setting up and running MD simulations
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Spatial and Temporal Localization of Connexins in Cells Using Confocal Microscopy,ing intercellular channels. These large-pore channels are dynamic and typically have a short life span of only a few hours. Imaging connexins from the time of synthesis in the endoplasmic reticulum through to their degradation can be challenging given their distinct assembly states and transient res
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Evaluation of Connexin Hemichannel Activity In Vivo,ive flow of small hydrophilic molecules and ions (≤1 kDa) between the cytosol and the extracellular environment. Activation of Cx HCs is highly dependent on pathological conditions. HC activity provokes changes in the microenvironment, inducing the dissemination of signaling molecules in both an aut
发表于 2025-3-23 08:46:50 | 显示全部楼层
Protocol for the Study of Connexin and DNA Interactions, of ions and molecules between the intra- and extracellular space and between cytoplasm of adjacent cells, respectively. The channel function of Cx assemblies has been extensively studied; however, “noncanonical” functions have emerged in the last few decades and have capture the attentions of many
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