Titlebook: Clinical Applications of PCR; Y. M. Dennis Lo,Rossa W. K. Chiu,K. C. Allen Chan Book 2006 Humana Press 2006 DNA.Laboratory.Mutation.PCR.ge

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Jolanta Chmielik,Natalia Grabarreaction tube. The fluorescence of either DNA dyes that are specific to double-strands or fluorescently labeled oligonucleotide probes can be monitored for both real-time quantification and melting analysis. When used together with rapid temperature control, these methods allow amplification and genotyping in less than a half hour.

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Real-Time Polymerase Chain Reaction and Melting Curve Analysis,reaction tube. The fluorescence of either DNA dyes that are specific to double-strands or fluorescently labeled oligonucleotide probes can be monitored for both real-time quantification and melting analysis. When used together with rapid temperature control, these methods allow amplification and genotyping in less than a half hour.

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Noninvasive Prenatal Diagnosis by Analysis of Fetal DNA in Maternal Plasma,te preanalytical handling of maternal blood samples, an efficient fetal DNA extraction protocol, and a sensitive and specific detection method. The protocol that has been applied regularly in the laboratory of the authors for the reliable detection and quantification of circulating fetal DNA is presented in this chapter.

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Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative is, it is further demonstrated that more than 80% of these DNA fragments are less than 180 bp in size. In this chapter, the clinical applications of plasma EBV DNA analysis and the protocols for the quantitative analysis of the size of circulating EBV DNA will be discussed.

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