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Titlebook: Clinical Applications of PCR; Y. M. Dennis Lo,Rossa W. K. Chiu,K. C. Allen Chan Book 2006 Humana Press 2006 DNA.Laboratory.Mutation.PCR.ge

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书目名称Clinical Applications of PCR
编辑Y. M. Dennis Lo,Rossa W. K. Chiu,K. C. Allen Chan
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Clinical Applications of PCR;  Y. M. Dennis Lo,Rossa W. K. Chiu,K. C. Allen Chan Book 2006 Humana Press 2006 DNA.Laboratory.Mutation.PCR.ge
描述In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.
出版日期Book 2006
关键词DNA; Laboratory; Mutation; PCR; gene expression
版次1
doihttps://doi.org/10.1385/159745074X
isbn_softcover978-1-61737-511-8
isbn_ebook978-1-59745-074-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2006
The information of publication is updating

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Jolanta Chmielik,Natalia Grabarbecome known as & quote;real-time& quote; PCR. Absolute quantification of initial template copy number can be obtained, although quantification relative to a control sample or second sequence is often adequate. Melting analysis following PCR monitors duplex hybridization as the temperature is change
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Gründe für die wahrscheinliche Entwicklungtwo analytes differ by 1 Da can be distinguished) and provides high sensitivity (less than 1 fmole of a DNA oligonucleotide can be detected), allowing DNA quantifications with single base specificity and single DNA molecule sensitivity (coupled with polymerase chain reaction [PCR]). To quantify a DN
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