Titlebook: Cilia; Methods and Protocol Vito Mennella Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Sc

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Frank Schimmelfennig,Guido Schwellnusy tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.

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Regierungssysteme in Mittel- und Osteuropase, and therapies. However, the primary basal cells required to establish the ALI cultures generally lose their ability to differentiate by the second or third passage, requiring a fresh batch, which can be limiting, particularly from donors with rare genotypes or in studies where gene modification

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Grotz Florian,Ferdinand Müller-Rommelom their apical surface into the cerebrospinal fluid (CSF). This specialized layer of E1 cells constitutes the border between the CSF and the brain interstitial fluid (BIF), and by controlling influx and efflux across the CSF to BIF interface, it is increasingly recognized to play an integral role i

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Regierungssysteme in Mittel- und Osteuropa fluid. In cultures, this ciliary beating is not well coordinated or occurs in small focal areas so the resulting mucociliary transport (MCT) is only linear over short distances. We present a method which induces ciliated cells in cultures to align during growth. The cells align along the axis of a

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SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Inteetter understanding respiratory pathogen host-cell interactions in the airways, one approach is to instead grow and differentiate these cells at an air-liquid interface (ALI). This chapter provides the working protocols used in our lab for producing ALI cultures, infecting them with SARS-CoV-2 and monitoring viral replication.

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Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging,living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.