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派博传思国际中心 »期刊书目

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Titlebook: Cilia; Methods and Protocol Vito Mennella Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Sc

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发表于 2025-3-21 19:33:45 | 显示全部楼层 |阅读模式
书目名称Cilia
副标题Methods and Protocol
编辑Vito Mennella
视频videohttp://file.papertrans.cn/227/226525/226525.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cilia; Methods and Protocol Vito Mennella Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Sc
描述.This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Cilia: Methods and Protocols. is a valuable resource for researchers who are interested in learning more about this developing field. .
出版日期Book 2024
关键词Tissue development; Homeostasis; Ciliopathies; Ciliary proteins; Human disease; Toxicant exposure
版次1
doihttps://doi.org/10.1007/978-1-0716-3507-0
isbn_softcover978-1-0716-3509-4
isbn_ebook978-1-0716-3507-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

书目名称Cilia影响因子(影响力)




书目名称Cilia影响因子(影响力)学科排名




书目名称Cilia网络公开度




书目名称Cilia网络公开度学科排名




书目名称Cilia被引频次




书目名称Cilia被引频次学科排名




书目名称Cilia年度引用




书目名称Cilia年度引用学科排名




书目名称Cilia读者反馈




书目名称Cilia读者反馈学科排名




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Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells,loped and optimized over the years to live image the centriole amplification dynamics. We explain why mouse brain MCC is a good model and provide the tips to enable successful spatially and temporally resolved monitoring of this massive organelle reorganization.
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Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice Using Endogenous Streptavi for the formation and function of all types of cilia. We describe a method to generate mice with an endogenous C-terminal streptavidin/FLAG tag, using . as an example, and demonstrate the successful implementation of AP in this model. This method can easily be adapted for N- and C-terminal tagging
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Zeitgeschichte und Politikwissenschafttinct deletions. We describe the use of CRISPR-Cas9 RNP in combination with single-cell RNA sequencing to functionally characterize the impact of gene invalidation in ALI cultures. The complex ecosystem of the airway epithelium, composed of many cell types, makes single-cell approaches particularly
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Pedagogy
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Regierungssystem und Verwaltungspolitikloped and optimized over the years to live image the centriole amplification dynamics. We explain why mouse brain MCC is a good model and provide the tips to enable successful spatially and temporally resolved monitoring of this massive organelle reorganization.
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Ulrich Widmaier,Andrea Gawrich,Ute Becker for the formation and function of all types of cilia. We describe a method to generate mice with an endogenous C-terminal streptavidin/FLAG tag, using . as an example, and demonstrate the successful implementation of AP in this model. This method can easily be adapted for N- and C-terminal tagging
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