Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2016 Springer Science+Business Media New York 2016 dynamic architecture.ch

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1064-3745 ation advice from the experts.This volume details a valuable collection of protocols andreviews, such as emerging experimental and theoretical approaches. Theseapproaches have resulted in a substantial improvement in the understanding ofchromosome architecture. .Chromosome Architecture:Methods and P

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https://doi.org/10.1007/978-3-476-02834-1y After Photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.

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https://doi.org/10.1007/978-3-663-12339-2ly via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and here we describe how flow cytometry can be used to assess the DNA content of . cells.

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Using Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Mainteny After Photobleaching (FRAP) as a method to study the properties of YFP-tagged MukB in fluorescent foci. This method can provide important insight into the dynamics of MukB on DNA and be used to study its biochemical properties in vivo.

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Imaging DNA Structure by Atomic Force Microscopy,re, we describe the application of AFM to determine superstructure and secondary structure of surface-bound DNA. The method is also readily applicable to probe DNA–DNA interactions and DNA–protein complexes.

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, Chromosome Copy Number Measurement Using Flow Cytometry Analysis,ly via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and here we describe how flow cytometry can be used to assess the DNA content of . cells.

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Visualizing , During Vegetative Growth and Spore Formation,e processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualize . during vegetative growth, and the developmental process of sporulation.

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Book 2016ulted in a substantial improvement in the understanding ofchromosome architecture. .Chromosome Architecture:Methods and Protocols. guides readers through cutting-edge interdisciplinarymethods which allow for an understanding of architecture of chromosomes withexceptionally enhanced resolution, both