Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2016 Springer Science+Business Media New York 2016 dynamic architecture.ch

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Book 2016ctive topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls..Authoritativeand cutting-edge,. .ChromosomeArchitecture: Methods and Protocols. .aims to ensure successful results in thefurther study of this vital field..

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Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localie freely or do both. We can study the numbers of proteins that interact with chromatin and also determine their residence time on chromatin. We can determine whether these proteins form functional clusters within the nucleus as well as whether they form specific nuclear structures.

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Investigating Bacterial Chromosome Architecture, I present a comprehensive methodology designed to characterize the architecture of the nucleoid DNA and the positioning of specific DNA sequences in live . cells. DNA localization systems, preparation of stable agarose-mounted microscopy slides, and basic image analysis tools are mentioned.

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In Vivo and In Situ Replication Labeling Methods for Super-resolution Structured Illumination Microls for highly efficient electroporation-based delivery or scratch loading of cell impermeable fluorescent nucleotides for live cell studies. Furthermore we describe the application of (2′S)-2′-deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU) for the in situ detection of segregated chromosome territories with minimized cytotoxic side effects.

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,DNA–Protein Interactions Studied Directly Using Single Molecule Fluorescence Imaging of Quantum Doteal-time fluorescence imaging of these interactions provides quantitative descriptions of search mechanism at the single molecule level. In our lab, we use this method to study the complex process of nucleotide excision DNA repair to determine mechanisms of damage detection, lesion removal, and DNA excision.