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Titlebook: Chromosome Architecture; Methods and Protocol Mark C. Leake Book 2016 Springer Science+Business Media New York 2016 dynamic architecture.ch

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Educational Writings in Chicago Yearstation, . undergoes the development process of spore formation, which is among the simplest examples of cellular differentiation. Many aspects of these processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualize . during v
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978-1-4939-8100-7Springer Science+Business Media New York 2016
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https://doi.org/10.1057/9780230592827as transcriptional regulation is fundamentally a single-molecule problem—a single repressor protein binding a single binding site in the genome can dramatically alter behavior at the whole cell and population level.
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Intra-Nuclear Single-Particle Tracking (I-SPT) to Reveal the Functional Architecture of Chromosomesthod to record the trajectories of weakly fluorescent molecules in the nucleus of living cells. I-SPT uses some specific detection and analysis tools to enable the computation of reliable statistics on nuclear particle movement.
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