Titlebook: Chromosomal Mutagenesis; Gregory D. Davis,Kevin J. Kayser Book 2008 Humana Press 2008 DNA.Eukaryotic organisms.Evolution.Gene disruption.G

轨道

Book 2008ions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls...Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types..

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带伤害

The Scandinavian Approach to mBankingation, and show that expression is reproducible among different targeted clones, and stable over a 4 mo period. These experiments were conducted with the natural yeast I-.I meganuclease, but the general design and process can also be applied to engineered meganucleases.

Gesture

https://doi.org/10.1007/978-3-642-10826-6e as indispensable tools in the genetic toolkit of vertebrate models. Here, we provide experimental protocols for the construction, functional testing, and application of the . transposon for insertional mutagenesis of the mouse germline.

tackle

Carmen Dobrovie-Sorin,Claire Beyssadele expression system in either slow- or fast-growing mycobacteria provides elevated recombination frequencies and facilitates simple allelic exchange using linear DNA substrates. Mycobacterial recombineering, therefore, offers a simple approach for constructing gene replacement mutants in . and ..

起波澜

栏杆

Robust Cell Line Development Using Meganucleases,ation, and show that expression is reproducible among different targeted clones, and stable over a 4 mo period. These experiments were conducted with the natural yeast I-.I meganuclease, but the general design and process can also be applied to engineered meganucleases.

美色花钱

Insertional Mutagenesis of the Mouse Germline With , Transposition,e as indispensable tools in the genetic toolkit of vertebrate models. Here, we provide experimental protocols for the construction, functional testing, and application of the . transposon for insertional mutagenesis of the mouse germline.

Graves’-disease

Mycobacterial Recombineering,le expression system in either slow- or fast-growing mycobacteria provides elevated recombination frequencies and facilitates simple allelic exchange using linear DNA substrates. Mycobacterial recombineering, therefore, offers a simple approach for constructing gene replacement mutants in . and ..

鸽子