Titlebook: Chromosomal Mutagenesis; Gregory D. Davis,Kevin J. Kayser Book 2008 Humana Press 2008 DNA.Eukaryotic organisms.Evolution.Gene disruption.G

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,Hope on the Horizon: Carter’s Election,es a simple antibiotic selection to isolate cell lines in which the introduced plasmid has integrated at the desired . site. A polymerase chain reaction assay is also presented to verify correct chromosomal placement of the introduced plasmid. This integration system based on ϕC31 integrase supplies

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1064-3745 mosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types..978-1-61737-833-1978-1-59745-232-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Book 2008 team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond mod

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Redefining Geometrical Exactnessonor DNA, allowing the experimenter to introduce designed sequence alterations. We describe the construction of ZFNs for novel targets and their application to targeted mutagenesis and targeted gene replacement in . and ..

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1064-3745 mat.Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homol

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Allelic Exchange of Unmarked Mutations in ,,. I describe below the challenges of allelic exchange in ., the overall mechanism of a suicide vector system, and how it can be used for efficient allelic exchange in this formally intractable organism.

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Gene Targeting in , and , With Zinc-Finger Nucleases,onor DNA, allowing the experimenter to introduce designed sequence alterations. We describe the construction of ZFNs for novel targets and their application to targeted mutagenesis and targeted gene replacement in . and ..