书目名称 | Chromosomal Mutagenesis | 编辑 | Gregory D. Davis,Kevin J. Kayser | 视频video | | 概述 | Highlights techniques involving a wider variety of cell types and organisms.Contains state-of-the-art methods in step-by-step laboratory format | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls...Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.. | 出版日期 | Book 2008 | 关键词 | DNA; Eukaryotic organisms; Evolution; Gene disruption; Gene knockouts; Gene therapy; Homologous recombinat | 版次 | 1 | doi | https://doi.org/10.1007/978-1-59745-232-8 | isbn_softcover | 978-1-61737-833-1 | isbn_ebook | 978-1-59745-232-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2008 |
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