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Titlebook: Chromosomal Mutagenesis; Gregory D. Davis,Kevin J. Kayser Book 2008 Humana Press 2008 DNA.Eukaryotic organisms.Evolution.Gene disruption.G

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发表于 2025-3-21 19:26:08 | 显示全部楼层 |阅读模式
书目名称Chromosomal Mutagenesis
编辑Gregory D. Davis,Kevin J. Kayser
视频video
概述Highlights techniques involving a wider variety of cell types and organisms.Contains state-of-the-art methods in step-by-step laboratory format
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chromosomal Mutagenesis;  Gregory D. Davis,Kevin J. Kayser Book 2008 Humana Press 2008 DNA.Eukaryotic organisms.Evolution.Gene disruption.G
描述.Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls...Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types..
出版日期Book 2008
关键词DNA; Eukaryotic organisms; Evolution; Gene disruption; Gene knockouts; Gene therapy; Homologous recombinat
版次1
doihttps://doi.org/10.1007/978-1-59745-232-8
isbn_softcover978-1-61737-833-1
isbn_ebook978-1-59745-232-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2008
The information of publication is updating

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Orpheus Recombination,sover recombination. In this way, desired clones can be specifically isolated from a heterogeneous library of phage. Furthermore, if the probe region contains a designed mutation, it may be deposited seamlessly into the genomic clone. The complete removal of operational sequences allows unlimited re
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Conditional Gene Trapping Using the FLEx System,gene synthesis but also in targeted and random mutagenesis have contributed to the current status. This chapter will highlight the milestones that have been undertaken in order to saturate the mouse genome with gene trap mutations. We have no intention to cover the entire field but will instead focu
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,Site-Specific Chromosomal Integration Mediated by ϕC31 Integrase,es a simple antibiotic selection to isolate cell lines in which the introduced plasmid has integrated at the desired . site. A polymerase chain reaction assay is also presented to verify correct chromosomal placement of the introduced plasmid. This integration system based on ϕC31 integrase supplies
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https://doi.org/10.1007/978-3-642-10826-6gene synthesis but also in targeted and random mutagenesis have contributed to the current status. This chapter will highlight the milestones that have been undertaken in order to saturate the mouse genome with gene trap mutations. We have no intention to cover the entire field but will instead focu
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Deindustrialisation in the New Millennium,reby generating Ty5 elements with new target specificities. The mechanism of Ty5 target site choice suggests that integration specificity of other retrotransposons and retroviruses can be altered by engineering integrases to recognize DNA-bound protein partners. Retroelements can also be used to pro
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