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Titlebook: Chromatin Protocols; Srikumar P. Chellappan Book 2009Latest edition Humana Press 2009 Chromatin immunoprecipitation (ChIP) assays.DNA.DNA

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书目名称Chromatin Protocols
编辑Srikumar P. Chellappan
视频video
概述Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques.Includes detailed, step by step guidelines for the analysis of chrom
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chromatin Protocols;  Srikumar P. Chellappan Book 2009Latest edition Humana Press 2009 Chromatin immunoprecipitation (ChIP) assays.DNA.DNA
描述.Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In .Chromatin Protocols, Second Edition., expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful .Methods in Molecular Biology™. series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls...Comprehensive and up-to-date, .Chromatin Protocols, Second Edition .is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology..
出版日期Book 2009Latest edition
关键词Chromatin immunoprecipitation (ChIP) assays; DNA; DNA repair and replication; Histones; Nucleosomes; Poly
版次2
doihttps://doi.org/10.1007/978-1-59745-190-1
isbn_softcover978-1-4939-5626-5
isbn_ebook978-1-59745-190-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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https://doi.org/10.1007/978-90-481-3156-3 have increased our understanding of the mechanism of catalysis of the PRMT family of enzymes. In the following discussion, we present some of the more commonly used in vivo and in vitro techniques which can be utilized to study the mechanism of arginine methylation and its role in transcription.
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Biochemical Analysis of Arginine Methylation in Transcription, have increased our understanding of the mechanism of catalysis of the PRMT family of enzymes. In the following discussion, we present some of the more commonly used in vivo and in vitro techniques which can be utilized to study the mechanism of arginine methylation and its role in transcription.
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Preparation of Chromatin Assembly Extracts from Preblastoderm , Embryos,e complexes of histones, histone chaperones and ATP-dependent nucleosome spacing factors. The resulting chromatin is an excellent approximation of physiological chromatin in vivo. This chapter describes the preparation of chromatin assembly extracts and the chromatin assembly reaction.
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Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strandheir position in the cell cycle and induction of apoptosis. This chapter presents the protocols and outlines applications of multiparameter cytometry in analysis of H2AX phosphorylation as a reporter of the presence of DSBs.
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