Titlebook: Chromatin Immunoprecipitation Assays; Methods and Protocol Philippe Collas Book 2009 Humana Press 2009 ChIP.DNA.DNA microarray.Genome-wide

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The State-of-the-Art of Chromatin Immunoprecipitation,on of ChIP with DNA microarray, paired-end ditag, and high-throughput sequencing technologies has in recent years enabled the profiling of histone modifications and transcription factor occupancy on a genome-wide scale. This review highlights the variations on the theme of the ChIP assay, the variou

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Characterization and Quality Control of Antibodies Used in ChIP Assays,er characterizations. Then, only specific antibodies are tested in ChIP using an optimized method which is ideal for antibody screening. Once QC is established for one antibody, it is used to similarly characterize each antibody batch in order to supply researchers in a reproducible manner with vali

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DamID: A Methylation-Based Chromatin Profiling Approach, methods and bioinformatics analysis (such as expression profiles and 5C analysis), DamID emerges as a powerful tool for analysis of chromatin structure and function in eukaryotes. DamID allows the detection of the direct genomic targets of any given factor independent of antibodies and without the

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Chromosome Conformation Capture (from 3C to 5C) and Its ChIP-Based Modification,e general protocol for 3C analysis with the subsequent estimation of ligation frequencies by using the real-time PCR technology with TaqMan probes. We discuss in details all steps of the experimental procedure paying special attention to weak points and possible ways to solve the problems. A special

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