Titlebook: Chromatin Immunoprecipitation Assays; Methods and Protocol Philippe Collas Book 2009 Humana Press 2009 ChIP.DNA.DNA microarray.Genome-wide

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Analysis of Nascent RNA Transcripts by Chromatin RNA Immunoprecipitation,is technique has a considerable margin of technological development especially in high-throughput screening experiments in combination with microarrays. In this chapter, we describe a RIP protocol optimized in our laboratory to study association of RNA binding proteins with specific nascent mRNA transcripts.

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Immunoprecipitation of Methylated DNA,te of interest can be detected by PCR, hybridization to DNA arrays, or by direct sequencing. This chapter describes the MeDIP protocol and quality control tests that should be performed throughout the procedure.

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Characterization and Quality Control of Antibodies Used in ChIP Assays,inly directed against targets relevant to the epigenetics field such as modified histones, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensiv

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The Fast Chromatin Immunoprecipitation Method,ctions involved in regulating gene expression, DNA repair, and cell division. The power of the assay is that it gives a researcher the ability to not only detect a specific protein–DNA interaction in vivo but also determine the relative density of factors along genes or the entire genome. Though pow

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,μChIP: Chromatin Immunoprecipitation for Small Cell Numbers,dified histones on DNA, often in relation to transcription or differentiation. Conventional ChIP protocols, however, require large number of cells, which limits the applicability of ChIP to rare cell samples. ChIP assays for small cell numbers (in the range of 10,000–100,000) have been recently repo