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Titlebook: Chromatin Immunoprecipitation Assays; Methods and Protocol Philippe Collas Book 2009 Humana Press 2009 ChIP.DNA.DNA microarray.Genome-wide

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发表于 2025-3-21 19:22:50 | 显示全部楼层 |阅读模式
书目名称Chromatin Immunoprecipitation Assays
副标题Methods and Protocol
编辑Philippe Collas
视频videohttp://file.papertrans.cn/227/226298/226298.mp4
概述Provides an up-to-date reference volume for chromatin immunoprecipitation assays written by leading researchers in the field.Delivers easily accessible and thoroughly tested bench protocols.Covers a w
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chromatin Immunoprecipitation Assays; Methods and Protocol Philippe Collas Book 2009 Humana Press 2009 ChIP.DNA.DNA microarray.Genome-wide
描述.Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In .Chromatin Immunoprecipitation Assays: Methods and Protocols., researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding know
出版日期Book 2009
关键词ChIP; DNA; DNA microarray; Genome-wide datasets; High-throughput sequencing; Protein binding; SNP; Terminat
版次1
doihttps://doi.org/10.1007/978-1-60327-414-2
isbn_softcover978-1-61779-750-7
isbn_ebook978-1-60327-414-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
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Désordres sévères de l’avant-piedlysis of ChIP-chip data very challenging. In this chapter, we review some of the issues involved in the analysis of ChIP-chip data and present a few statistical methods that can be used to overcome these issues and improve the detection of DNA–protein binding sites.
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Chen Peng,Chuanliang Cheng,Ling Wangecipitation of histone proteins or transcription factors under cross-linking conditions. We describe here a rapid micro (μ)ChIP assay suited for multiple parallel ChIPs from a single chromatin batch from 1,000 cells. The assay is also applicable to a single immunoprecipitation from 100 cells.
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发表于 2025-3-22 15:44:44 | 显示全部楼层
Désordres sévères de l’avant-pieder, such expression is no longer endogenous. To surmount this problem, we have successfully developed a facile method to knock in a 3xFlag epitope into the endogenous gene loci of transcription factors. The knock-in approach provides a general solution for the study of proteins for which antibodies are substandard or not available.
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Quelques pathologies du 5e rayonation of human myoblasts into myotubes. The findings have been validated by the observation of a significant correlation between the detected histone modifications and the expression of the nearby genes, as measured by DNA expression microarrays. This chapter focuses on the computational analysis of the data.
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Epitope Tagging of Endogenous Proteins for Genome-Wide Chromatin Immunoprecipitation Analysis,er, such expression is no longer endogenous. To surmount this problem, we have successfully developed a facile method to knock in a 3xFlag epitope into the endogenous gene loci of transcription factors. The knock-in approach provides a general solution for the study of proteins for which antibodies are substandard or not available.
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