Titlebook: Chromatin; Methods and Protocol Julia Horsfield,Judith Marsman Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive l

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State Renewal in Africa: The Lessonsng histone modifications and DNA–protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

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https://doi.org/10.1057/978-1-137-58782-4nd their complexes have been identified before, but how each genomic locus interacts with its surrounding proteins in the nucleus over time and in changing conditions remains poorly described. Measuring protein–DNA interactions at a specific locus in the genome is challenging and current techniques

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‘Education’ and the Pruning of the Mindd as a powerful method to quantify the dynamics of nuclear proteins such as transcription factors (TFs). Here, we provide a protocol for conducting and analyzing SPT experiments with a focus on fast tracking (“fastSPT”) of TFs in mammalian cells. First, we explore how to engineer and prepare cells f