Titlebook: Chromatin; Methods and Protocol Julia Horsfield,Judith Marsman Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive l

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Tdd526

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unstable-angina

Nanopore Sequencing and Data Analysis for Base-Resolution Genome-Wide 5-Methylcytosine Profilinge nature of sodium bisulfite results in DNA fragmentation and subsequent biases in sequencing data. Such issues have led to the development of bisulfite-free methods for 5mC detection. Nanopore sequencing is a long read nondestructive approach that directly analyzes DNA and RNA fragments in real tim

砍伐

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cng histone modifications and DNA–protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

modest

A Robust Protocol for Investigating the Cohesin Complex by ChIP-SequencingCTCF collaborate to form chromatin loops and to gain insight in the intricate regulation of cohesin. Here we describe a detailed ChIP protocol that has been successfully used for different cohesin subunits and cohesin regulators in various cell lines.

antipsychotic

Epi-Decoder: Decoding the Local Proteome of a Genomic Locus by Massive Parallel Chromatin Immunoprecnd their complexes have been identified before, but how each genomic locus interacts with its surrounding proteins in the nucleus over time and in changing conditions remains poorly described. Measuring protein–DNA interactions at a specific locus in the genome is challenging and current techniques

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A Protocol for Studying Transcription Factor Dynamics Using Fast Single-Particle Tracking and Spot-Od as a powerful method to quantify the dynamics of nuclear proteins such as transcription factors (TFs). Here, we provide a protocol for conducting and analyzing SPT experiments with a focus on fast tracking (“fastSPT”) of TFs in mammalian cells. First, we explore how to engineer and prepare cells f

Compass

Characterization of Mammalian Regulatory Complexes at Single-Locus Resolution Using TINCresponsible for cellular identity. Consequently, insight into the molecular composition of these regulatory complexes is of major importance for our understanding of any physiological or pathological cellular state or transition. However, it remains extremely difficult to identify the protein comple

organism

Profiling Protein–DNA Interactions Cell-Type-Specifically with Targeted DamIDolymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bio