Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Recording of Chromaffin Cell Electrical Activity In Situ in Acute Adrenal Slicesal step in assessing the adrenal medullary tissue function. The difficult access to the gland in vivo allows only population activity to be recorded in this condition. In vitro preparations allow recordings of spontaneous or evoked activity from single or multiple cells, depending on the biological

JADED

Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normmine (CA) release reported in adrenal glands from adult SHRs raised considerable interest for its possible implication in the genesis of hypertension. The use of powerful techniques such as calcium imaging, electrophysiology, and single-cell amperometry to monitor in real time the key steps in CA se

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Quantification of Secretory Granule Exocytosis by TIRF Imaging and Capacitance Measurementsmembrane fuses with the plasma membrane, which allows release of the stored content into the bloodstream or the surrounding tissue. Here, we give a detailed description of two complementary methods to observe and quantify exocytosis in single cells: high-resolution TIRF microscopy and patch-clamp ca

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Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Releasedir application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluat

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Immunogold for Protein Location in Chromaffin Cellsbedding immunogold enables the simultaneous localization of different molecules in the cell using secondary antibodies conjugated with gold particles of different size. In this chapter, we introduce pre-embedding and post-embedding immunogold procedures used for the identification of quantitative ch

Contort

Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normsiological relevance. Here, we describe the procedures to measure the blood pressure of rats in a noninvasive manner, how to obtain primary cultures of adrenal chromaffin cells and acute adrenal slices, and how to perform amperometric recordings and intracellular calcium imaging in these preparation