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Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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书目名称Chromaffin Cells
副标题Methods and Protocol
编辑Ricardo Borges
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to
描述.This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.. ..Authoritative and cutting-edge, .Chromaffin Cells: Methods and Protocols .aims to be a useful practical guide to researches to help further their study in this field. .
出版日期Book 2023
关键词Immunostaining; Electron microscopy; PC12 cells; electroporation; CRISPR
版次1
doihttps://doi.org/10.1007/978-1-0716-2671-9
isbn_softcover978-1-0716-2673-3
isbn_ebook978-1-0716-2671-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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978-1-0716-2673-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Chromaffin Cells978-1-0716-2671-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-3-319-68622-6cytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.
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https://doi.org/10.1007/978-3-319-68622-6cytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity liga
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Leonid Grinin,Sergey Malkov,Andrey Korotayevisadvantages to the use of cultured cells for immunocytochemistry. One of the advantages is that cultured cells can be used for one or more weeks after the dissociation of cells, whereas one of the disadvantages is that the properties of cells in culture might change under artificial conditions. On
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