Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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https://doi.org/10.1057/9780230339378in their molecule chemical groups with the ability to yield (oxidize) or capture (reduce) electrons upon its exposure to an electrical field. In order to measure the secretion of catecholamines, they are oxidized at +650 mV with a carbon electrode, releasing every molecule of catecholamine that is o

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https://doi.org/10.1057/9780230339378al step in assessing the adrenal medullary tissue function. The difficult access to the gland in vivo allows only population activity to be recorded in this condition. In vitro preparations allow recordings of spontaneous or evoked activity from single or multiple cells, depending on the biological

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Liberalism and the Challenge of Pluralismmine (CA) release reported in adrenal glands from adult SHRs raised considerable interest for its possible implication in the genesis of hypertension. The use of powerful techniques such as calcium imaging, electrophysiology, and single-cell amperometry to monitor in real time the key steps in CA se

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https://doi.org/10.1007/978-1-349-27445-1ation and shaping of the local and global cytosolic Ca. signals triggering the process of regulated exocytosis in chromaffin cells. Beyond that role, intraorganellar Ca. homeostasis also regulates organelle-specific processes such as oxidative phosphorylation in mitochondria, maturation of secretory

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Liberalism and the Challenge of Pluralismmembrane fuses with the plasma membrane, which allows release of the stored content into the bloodstream or the surrounding tissue. Here, we give a detailed description of two complementary methods to observe and quantify exocytosis in single cells: high-resolution TIRF microscopy and patch-clamp ca

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Reconstituting Social Criticismling regulated exocytosis. This is in part due to the exquisite temporal resolution of the technique, and the possibility of combining quantification of exo-/endocytosis at the whole-cell level, with the ability to simultaneously monitor and control the calcium signals triggering vesicle fusion. In

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Philosophy, Contingency and Social Criticism, or serotonin, released by exocytosis from secretory cells..Amperometry has two main characteristics that make it ideal for the study of exocytosis at the single-cell level with single-vesicle resolution quantal release. (i) .. The carbon fiber microelectrode can be carefully positioned on plasma m

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https://doi.org/10.1007/978-3-642-21572-8ir application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluat

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Rat Pheochromocytoma PC12 Cells in Culturecytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.