Titlebook: Chlamydia trachomatis; Methods and Protocol Amanda Claire Brown Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 201

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A Coinfection Model to Evaluate Chlamydia Inc Protein Interactions,eins (Inc), are embedded within the inclusion membrane and facilitate the interaction of the inclusion with host cell organelles. These interactions are vital for bacterial replication and allow for the acquisition of essential nutrients from the host cell. However, it is not known if Inc proteins f

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Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy, culture, nonculture methods, including detection of antigen or DNA, are not able to differentiate between viable and nonviable organisms. As an obligate intracellular bacterium, . replicates inside host cells by forming unique organelles called inclusions. Here, we describe the enumeration of viabl

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Proximity Labeling of the , Inclusion Membrane,transmembrane or membrane-associated proteins are organized and function within the membranes of these pathogen-containing vacuoles. Yet this host–pathogen interaction interface has proven difficult to experimentally resolve. For example, one method to begin to understand protein function is to dete

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High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, Including , enhance host cell control of pathogens, which can then go on to be developed as novel therapeutics. Typically screening is commonly done in artificial culture medium; however, obligate intracellular pathogens, such as ., cannot be tested this way. Intracellular screening methods allow for such path

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Whole-Genome Sequencing of , Directly from Human Samples, nonmutagenic approach, we can capture all known variation found within . genomes. The method is a consistent and sensitive tool that enables rapid whole-genome sequencing of . directly from clinical samples and has the potential to be adapted to other pathogens with a similar clonal nature.

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Tim Stüdemann,Florian Weinberger NG. Since the method is performed at low and constant temperature, it can therefore be run on portable instruments. SIBA enables rapid screening for CT and NG within point-of-care or central laboratory settings.