Titlebook: Chlamydia trachomatis; Methods and Protocol Amanda Claire Brown Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 201

Thoracic

书目名称Chlamydia trachomatis影响因子(影响力)




书目名称Chlamydia trachomatis影响因子(影响力)学科排名




书目名称Chlamydia trachomatis网络公开度




书目名称Chlamydia trachomatis网络公开度学科排名




书目名称Chlamydia trachomatis被引频次




书目名称Chlamydia trachomatis被引频次学科排名




书目名称Chlamydia trachomatis年度引用




书目名称Chlamydia trachomatis年度引用学科排名




书目名称Chlamydia trachomatis读者反馈




书目名称Chlamydia trachomatis读者反馈学科排名

CRAMP

Engaging

新手

Antimicrobial Resistance Screening in , by Optimized McCoy Cell Culture System and Direct qPCR-Basey. Cell culture systems with immunofluorescence staining, to identify cellular inclusions in the presence of various concentrations of antimicrobial drugs, are still the most pervasive techniques, but more specific and sensitive nucleic acid concentration measuring methods are increasingly being use

里程碑

Whole-Genome Sequencing of , Directly from Human Samples,hy and mutations associated with antimicrobial resistance. The ability to sequence pathogen genomes directly from clinical specimens, without the requirement for in vitro culturing, is attractive in terms of time- and labor-saving, especially in the case of slow growing, or obligate intracellular pa

Anecdote

Multilocus Sequence Typing (MLST) of ,y globally adopted as a universal fine-detailed molecular typing tool and has been applied to numerous pathogenic and nonpathogenic bacterial as well eukaryotic organisms. MLST utilizes DNA sequence of several conserved housekeeping (HK) genes which are assigned an allelic number, which then collect

Anecdote

轻快来事

Dual RNA-Seq of Chlamydia and Host Cells,onds to the other via defense or survival strategies. Unraveling this complex interplay is key for our understanding of bacterial virulence and host response pathways for the development of novel therapeutics. Dual RNA sequencing (dual RNA-Seq) has recently been developed to simultaneously capture h

暴发户

Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection,dissection enables isolation of single live cells. Here we describe a method for the isolation of single .-infected cells using a laser microdissection system, in which the dissected samples are captured via gravity. Cells infected by . at low multiplicity of infection are marked with the fluorescen

天然热喷泉

Genetic Manipulation of ,: Chromosomal Deletions,pulation of .. Valuable approaches such as random, chemically induced mutagenesis or targeted, insertion-based gene disruption have led to significant discoveries. We describe herein a technique for generating definitive null strains via complete deletion of chromosomal genes in .. Fluorescence-repo