Titlebook: Chlamydia trachomatis; Methods and Protocol Amanda Claire Brown Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 201

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Cindy Kok,Dhanya Ranvindran,Eddy Kizanalt can be confirmed by UV spectra and agarose gel electrophoresis. This assay uses all nonhazardous chemical reagents and is hence safe to the users, and requires little specialist training or knowledge.

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Lecture Notes in Electrical Engineeringny bacteria–eukaryotic host interaction. We pioneered dual RNA-Seq to simultaneously capture . and host expression profiles during an in vitro infection as proof of principle. Here we provide a detailed laboratory protocol and bioinformatics analysis guidelines for dual RNA-seq experiments focusing on . as the organism of interest.

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Mirza Šarić,Jasna Hivziefendić,Lejla Bandić here describes steps required to produce transformation competent chlamydiae, generate a specific allelic exchange plasmid construct, carry out mutagenesis, and isolate clonal populations of resulting mutant strains.

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Benford’s Law and Sum Invariance Testingnteractions of Inc proteins through the coinfection of . strains expressing differently tagged inclusion membrane proteins. Our approach takes advantage of the natural homotypic fusion of inclusions and allows for the study of Inc protein interactions when they are embedded within the inclusion membrane.

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Detection of , and , Using Multiplex Strand Invasion Based Amplification (mSIBA), NG. Since the method is performed at low and constant temperature, it can therefore be run on portable instruments. SIBA enables rapid screening for CT and NG within point-of-care or central laboratory settings.

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Point-of-Care , Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue,lt can be confirmed by UV spectra and agarose gel electrophoresis. This assay uses all nonhazardous chemical reagents and is hence safe to the users, and requires little specialist training or knowledge.

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Dual RNA-Seq of Chlamydia and Host Cells,ny bacteria–eukaryotic host interaction. We pioneered dual RNA-Seq to simultaneously capture . and host expression profiles during an in vitro infection as proof of principle. Here we provide a detailed laboratory protocol and bioinformatics analysis guidelines for dual RNA-seq experiments focusing on . as the organism of interest.

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