Titlebook: Chemokine Protocols; Amanda E. I. Proudfoot,Timothy N. C. Wells,Christi Book 2000 Humana Press 2000

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Modified Microchemotaxis Assays,motactic molecules. Upon encountering a chemotactic molecule, responding leukocytes begin to migrate directionally from regions of low ligand concentrations toward the sites of chemoattractant production that typically possess more substantial levels of soluble chemotactic factors. A number of endog

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Transwell Chemotaxis,in activation, chemotaxis, lipid mediator biosynthesis, superoxide radical production, and granule enzyme release (reviewed in ..–.), chemokines have been shown to suppress and stimulate angiogenesis (.–.), suppress hematopoiesis (.–.), suppress apoptosis (.), control viral infection (.,.), and effe

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Radiolabeled Chemokine Binding Assays,ine system represents a striking example as most receptors in the family bind multiple chemokines, often with complex overlapping selectivities. Two basic methodologies are used to characterize the binding properties of a receptor. In saturation protocols, the binding of a given ligand, usually labe

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Scintillation Proximity Binding Assay,urthermore, receptor-ligand binding assays are used extensively for drug screening. The Scintillation Proximity Assay (SPA) is now a well established screening technology and a considerable number of SPA based receptor screening assays have been run by pharmaceutical companies. This homogeneous radi

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Calcium Mobilization,gen, sodium, and potassium concentrations. It is generally recognized that cells spend a great amount of their energy on stringent regulation of the level of cytosolic free Ca.+, and that Ca.+ plays a very important regulatory role in cellular functions

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Actin Polymerization, of cells into the inflamed tissue, they have to pass the endothelium. This process involves several types of adhesion molecule interactions between eosinophils and endothelial cells. Selectins mediate two steps, initial tethering to the vessel wall and their subsequent rolling. Integrins then bind

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Histamine Release,tory role in immediate hypersensitivity reactions. Some of the mediators are preformed and stored in granules. An example is histamine. Other mediators are generated ., e.g., eicosanoids. Mast cells and basophils produce some important cytokines, e.g., IL-4, IL-13, TNF-α (.–.) and the chemokines MlP

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