Titlebook: Chemical Protein Synthesis; Xuechen Li Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Scie

Temporal-Lobe

https://doi.org/10.1007/978-1-4302-0310-0led procedures for the synthesis of Fmoc-protected SetCys residue and for its incorporation into peptides using standard solid-phase peptide synthesis protocols. We also describe its use for the chemical synthesis of proteins through the redox-controlled assembly of three peptide segments in one-pot.

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一美元

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Obverse

附录

Continuous-Flow Synthesis of ,-Methylated Peptides via Generation of an Acyl ,-Methylimidazolium Caent amounts of amino acids. The addition of a strong Brønsted acid is critical to generate the highly reactive .-methylimidazolium cation species to accelerate the amidation. The developed approach enabled the synthesis of a bulky peptide with a higher yield in a shorter amount of time compared with the results of conventional amidation.

Malleable

,Covalent Reactive Peptides to Block Protein–Protein Interactions and Inhibit Microbe–Host Interactihost cells instead of bacterium cells. Here we present the experimental details of the design and synthesis of cysteine-reactive peptides to selectively block Nck-SH3.2 but not the other two SH3 domains. Procedures of EPEC infection, covalent reaction inside Caco-2 cells, and bacterial counting to check the antibacterial effect are also described.

摇曳

昏睡中

Side-Chain Anchoring Strategies for the Synthesis of Peptide Thioesters and Selenoesters,y. Importantly, this methodology overcomes solubility issues and epimerization of the C-terminal amino acid residue that can occur using solution-phase approaches. Detailed methods for the solid-phase synthesis of peptide thioesters and selenoesters using a side-chain anchoring approach are outlined in this article.