Titlebook: Checkpoint Controls and Cancer; Volume 2: Activation Axel H. Schönthal Book 2004 Humana Press 2004

Antimicrobial

Analysis of Checkpoint Responses to Histone Deacetylase Inhibitorsinduced by genotoxic agents that cause DNA damage. Histone deacetylase inhibitors not only increase the acetylation state of chromatin histones, but they also perturb the cell cycle, causing both G1 and G2 phase arrests, the latter by initiating a checkpoint response. In this chapter we will describ

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Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Analect p53 target genes. Taqman® analysis and quantitative chromatin immunoprecipitation analysis are used to validate the . prediction and microarray data. Enrichment factor analysis is used to demonstrate that . prediction greatly enriches for genes that are transcriptionally regulated by p53 and ass

中国纪念碑

certain

Analysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Responsef p21 recruitment, as well as for protein-protein interaction studies. An . extraction protocol is also described for immunofluorescence microscopy and flow cytometric analyses of nuclear localization and cell cycle distribution of p21 recruited to DNA repair foci. The combination of these methodolo

Obvious

Quantitative Determination of p16 Gene Expression by RT-PCRa laser integrated in the sequence detector. This chapter will cover the isolation of total RNA from frozen tissue, the transcription of RNA to cDNA, and the analysis of the relative gene expression by qRT-PCR. Although in principle applicable to any gene of interest, we will use as an example the q

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Measuring Cyclin-Dependent Kinase Activitypolyacrylamide gel and exposing it to film. Here, I describe the execution of this in vitro kinase activity assay as well as appropriate controls that need to be considered for the proper evaluation of the results.