Titlebook: Checkpoint Controls and Cancer; Volume 2: Activation Axel H. Schönthal Book 2004 Humana Press 2004

闷热

不遵守

Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle to study the cell cycle-dependent dephosphorylation of pRb by various PP1 isozymes, the specificity of PP1 isozymes for distinct pRb phosphorylation sites, the dephosphorylation of pRb associated with apoptosis, and the cell cycle- and pRb-dependent phosphorylation of PP1.

内向者

CUMB

Analyzing the Regulation and Function of ATMd with anti-ATM antibodies, and protein kinase activity is measured using p53.-specific substrate or by immunoblotting extracts with an anti-phosphoserine 15 p53-specific antibody. Missense mutations affecting ATM kinase activity are detected using in vitro mutagenesis of ATM cDNA followed by the procedures outlined above.

纬度

Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells. We report here a protocol for purification of an inhibitor of the APC/C from HeLa cells, called mitotic checkpoint complex (MCC). Our procedure is based on biochemical purification and characterization of the APC/C inhibitory activity from extracts of HeLa cells.

instate

Analysis of the Spindle-Assembly Checkpoint in HeLa Cellses and spindle tension, chromosome positioning, and kinetochore signaling by the Mad2 or Bub1 checkpoint proteins. We also describe a bi-parameter flow cytometric assay, using either MPM-2 or anti-phospho-(Ser10)-histone H3 antibodies, for quantitating mitotic cells.

DOLT

Introduction to Flex Applications, to study the cell cycle-dependent dephosphorylation of pRb by various PP1 isozymes, the specificity of PP1 isozymes for distinct pRb phosphorylation sites, the dephosphorylation of pRb associated with apoptosis, and the cell cycle- and pRb-dependent phosphorylation of PP1.

神刊

Introduction to Flex Applications,in immune-complex kinase assays. This assay involves isolation of the kinase by immunoprecipitation and the in vitro phosphorylation of a specific substrate in the presence of radio-labeled ATP. Here we describe, in detail, the determination of PIKK catalytic activity with a standardized immune-complex kinase assay protocol.

Friction

https://doi.org/10.1007/978-1-4302-1836-4d with anti-ATM antibodies, and protein kinase activity is measured using p53.-specific substrate or by immunoblotting extracts with an anti-phosphoserine 15 p53-specific antibody. Missense mutations affecting ATM kinase activity are detected using in vitro mutagenesis of ATM cDNA followed by the procedures outlined above.

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