Titlebook: Cellular Quiescence; Methods and Protocol H. Daniel Lacorazza Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018

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Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches,

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Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Ceuorescein succinimidyl ester (CFSE) staining and flow cytometric analysis. Quiescent glioblastoma cells with stem-like features are characterized and subsequently isolated based on their ability to retain the CFSE labeling.

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Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq andand loss of hallmarks of quiescence. Thus, in vitro models that recapitulate the dynamic reversibly arrested stem cell state in culture and lend themselves to comparison with the activated or differentiated state are useful templates for genome-wide analysis of the quiescence network..In this chapte

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Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in QuiescentG0 leukemic cells. Nucleofection of a microRNA target reporter and either cognate, targeting microRNA, or control microRNA, into the nucleus of G0 cells, enables analysis of translation activation by microRNAs in G0. We discuss a modified protocol that we developed for transfection of mRNAs along wi

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Genome-Wide Identification of Transcription Factor-Binding Sites in Quiescent Adult Neural Stem Cel the genome can be obtained by assessing DNA-protein interaction through next-generation chromatin immunoprecipitation sequencing technology (ChIP-seq). This chapter outlines the protocol to perform, analyze, and validate ChIP-seq experiments that can be used to identify protein-DNA interactions and

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1064-3745 ndtips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Cellular Quiescence: Methods and Protocols. offers a broad view of basic and cutting-edge technology to inspire research in t978-1-4939-8465-7978-1-4939-7371-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Anatomy and Physiology of the Larynxuorescein succinimidyl ester (CFSE) staining and flow cytometric analysis. Quiescent glioblastoma cells with stem-like features are characterized and subsequently isolated based on their ability to retain the CFSE labeling.

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