Titlebook: Cellular Quiescence; Methods and Protocol H. Daniel Lacorazza Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018

maladorit

Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence,ent regulation of quiescence has also been implicated in the differentiation and function of memory T cells. In this chapter, we describe techniques to assess quiescent state of naïve T cells under steady state and exit from quiescence upon TCR stimulation.

不在灌木丛中

osteopath

Pathological Conditions of the Vocal Foldong-lived GFP label retaining cells. This method is free from confounding factors of previous methodologies based on radioactive tracers and also enables functional studies not previously possible using the radioactive tracer techniques described in the literature.

uveitis

Cardioversion

Anatomy and Physiology of the Larynxed tet-regulatable transgenic mouse model can be used to express histone H2B-GFP in epithelial proliferative cells and their dilution and retention of the GFP signal can be followed. In this chapter, we detail the steps to perform BrdU pulse-chase and H2B-GFP pulse-chase experiments to identify quiescent cells in the hair follicle.

轻率看法

节约

Palpitation

Cell Cycle Analysis by Mass Cytometry,es. The method utilizes incorporation of 5-Iodo-2′-deoxyuridine (IdU), combined with measurement of phosphorylated retinoblastoma protein (pRb), cyclin B1, and phosphorylated histone H3 (p-HH3). These measurements can be integrated into a gating strategy that allows for clear separation of all five phases of the cell cycle.

下边深陷

Identifying Quiescent Stem Cells in Hair Follicles,ed tet-regulatable transgenic mouse model can be used to express histone H2B-GFP in epithelial proliferative cells and their dilution and retention of the GFP signal can be followed. In this chapter, we detail the steps to perform BrdU pulse-chase and H2B-GFP pulse-chase experiments to identify quiescent cells in the hair follicle.

尖酸一点

A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Micros. We successfully screened for compounds that selectively eliminated cancer cells versus supportive stromal cells and verified results with comparison to efficacy in vivo. The spheroid coculture system successfully modeled key aspects of DTCs in the bone marrow microenvironment, facilitating testing for compounds to selectively eliminate DTCs.