书目名称 | Cellular Quiescence | 副标题 | Methods and Protocol | 编辑 | H. Daniel Lacorazza | 视频video | | 概述 | Includes cutting-edge methods and protocols for the study of cellular quiescence.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the e | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Cellular Quiescence: Methods and Protocols. offers a broad view of basic and cutting-edge technology to inspire research in t | 出版日期 | Book 2018 | 关键词 | Cellular dormancy; Stem cells; Prokaryotes; Eukaryotes; Genomic regulation | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-7371-2 | isbn_softcover | 978-1-4939-8465-7 | isbn_ebook | 978-1-4939-7371-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2018 |
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