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Titlebook: Cell-free DNA as Diagnostic Markers; Methods and Protocol Valentina Casadio,Samanta Salvi Book 2019 Springer Science+Business Media, LLC, p

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From cfDNA to Sequencing: Workflows and Potentialslp to accurately detect somatic mutations at low frequency. Here, we report some advantages and limitations of NGS approaches for cfDNA mutation analyses with the aim of choosing the most suitable in terms of sensitivity, specificity, data output, costs, and time work.
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Observer-Based Fault Estimation Techniquesnitoring, clinical decision, and patients’ outcome..cfDNA has already given a huge impact on prenatal medicine, and it could become, in the next future, the standard of care also in other fields, from oncology to transplant medicine and cardiovascular diseases.
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Foteini Cheirdari,George Karabatise consists of a pair of primers and two probes labeled with a fluorescent dye capable to recognize the presence or absence of a specific mutation. Using the dPCR method, we can identify only a few mutations in tissue that are present also in plasma.
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Cell-Free DNA: Applications in Different Diseasesnitoring, clinical decision, and patients’ outcome..cfDNA has already given a huge impact on prenatal medicine, and it could become, in the next future, the standard of care also in other fields, from oncology to transplant medicine and cardiovascular diseases.
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