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Titlebook: Cell-free DNA as Diagnostic Markers; Methods and Protocol Valentina Casadio,Samanta Salvi Book 2019 Springer Science+Business Media, LLC, p

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书目名称Cell-free DNA as Diagnostic Markers
副标题Methods and Protocol
编辑Valentina Casadio,Samanta Salvi
视频videohttp://file.papertrans.cn/223/222955/222955.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell-free DNA as Diagnostic Markers; Methods and Protocol Valentina Casadio,Samanta Salvi Book 2019 Springer Science+Business Media, LLC, p
描述.This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Authoritative and cutting-edge, .Cell-Free DNA as Diagnostic Markers: Methods and Protocols .aims to ensure successful results in the further study of this vital field..
出版日期Book 2019
关键词cancer; Urinary cell free DNA; Bioinformatics; DNA integrity; Mutations analyses
版次1
doihttps://doi.org/10.1007/978-1-4939-8973-7
isbn_ebook978-1-4939-8973-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Cell-Free DNA Integrity: Applicationsen studied for cfDNAi, such as specific genes involved in cancer progression or repetitive DNA sequences as surrogate markers for the whole circulating DNA..However, the lack of a standardized method for cfDNAi evaluation remains one of its main critical issues. Apoptotic index (AI) and integrity in
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Studying Copy Number Variations in Cell-Free DNA: The Example of AR in Prostate Cancerrting from the hypothesis that the gain of androgen receptor (.) gene is a frequent aberration in advanced prostate cancer patients, we analyzed it in cfDNA as a potential predictive biomarker of specific treatments. Here we report a general protocol that may be considered to analyze gene copy numbe
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From cfDNA to Sequencing: Workflows and Potentialsesistance by following the mutation status changes of patients. In this field, high-sensitivity methods like next-generation sequencing (NGS) could help to accurately detect somatic mutations at low frequency. Here, we report some advantages and limitations of NGS approaches for cfDNA mutation analy
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