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Titlebook: Cell‘Cell Interactions; Methods and Protocol Sean P. Colgan Book 2006 Humana Press 2006 bacteria.biology.biosynthesis.cell.cloning.membrane

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书目名称Cell‘Cell Interactions
副标题Methods and Protocol
编辑Sean P. Colgan
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell‘Cell Interactions; Methods and Protocol Sean P. Colgan Book 2006 Humana Press 2006 bacteria.biology.biosynthesis.cell.cloning.membrane
描述A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
出版日期Book 2006
关键词bacteria; biology; biosynthesis; cell; cloning; membrane; metabolism; microscopy; migration; molecular biolog
版次1
doihttps://doi.org/10.1385/1597451134
isbn_softcover978-1-61737-618-4
isbn_ebook978-1-59745-113-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2006
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Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization,The interactions between egg and sperm are among the most fascinating in cell biology. These interactions include Cell-Cell adhesion and then membrane fusion between the two gametes. This chapter details the experimental methods used to for gamete culture and in vitro fertilization using mouse sperm and eggs.
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Okinawa and Jeju: Bases of Discontentat brain endothelial cell line 4 (RBE4) express many properties that are expressed by brain endothelial cells in vivo. Tissue culture methods allow the investigator to design experiments for studying transporters and permeability that would be much more difficult in vivo. A method for making prepara
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Okinawa and Jeju: Bases of Discontential cells (IECs) represent an important barrier between lamina propria cells and the potentially harmful lumenal contents. In addition, IECs are important immunoeffector cells with the capacity to release cytokines, chemokines, and other molecules involved in antigen presentation and immune defense.
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