Titlebook: Cell‘Cell Interactions; Methods and Protocol Sean P. Colgan Book 2006 Humana Press 2006 bacteria.biology.biosynthesis.cell.cloning.membrane

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Collagen Gel Contraction Assay,hmic pattern of contractions controlled by its intrinsic neuronal innvervation. In chronic inflammatory diseases such as Crohn’s disease, the intestine can become stiff and fibrotic, losing much of its normal motility. Although muscle fiber contraction accounts for much of this activity, contraction

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,Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesibiofilm formation in the pathogenesis of certain types of infection, especially those involving indwelling medical devices, is becoming increasingly apparent. Critical to the development of a biofilm is the elaboration of exopolysaccharide that contributes to substrate and intercellular adhesion. Th

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A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplaenterotoxin produced by the bacterium .. The toxin must enter the intestinal cell to cause disease. Entry is achieved by the B-subunit binding to a membrane lipid that carries the toxin all the way from the plasma mem-brane through the .-Golgi to the endoplasmic reticulum (ER). Once in the ER, a por

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Expression Cloning of Signaling Proteins Regulated by Cell Adhesion,ation. Adhesion-related signal transduction is important throughout development, hemostasis, immunity, and in diseases such as cancer. Therefore, the identification of the various signaling pathways that are involved is crucial. Expression cloning is an unbiased way to isolate proteins with specific

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Assays for the Calcium Sensitivity of Desmosomes,ogenesis, or if they are taking part in invasion and metastasis. Desmosomes are one of the principal types of adhesive junctions in epithelia and are responsible for maintaining tissue integrity. It is likely that modulation of desmosomal adhesion is required to facilitate cell motility in response

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,Tight Junctions and Cell–Cell Interactions,ecrosis factor-α. Increased mucosal levels of these cytokines have been observed in mucosal biopsies from patients with a chronic inflammatory condition referred to as inflammatory bowel disease. Paracellular permeability across epithelial cells is regulated by tight junctions (TJ.), which are the a

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