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Titlebook: Cell-Based Assays Using iPSCs for Drug Development and Testing; Carl-Fredrik Mandenius,James A. Ross Book 2019 Springer Science+Business M

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书目名称Cell-Based Assays Using iPSCs for Drug Development and Testing
编辑Carl-Fredrik Mandenius,James A. Ross
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell-Based Assays Using iPSCs for Drug Development and Testing;  Carl-Fredrik Mandenius,James A. Ross Book 2019 Springer Science+Business M
描述The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Authoritative and cutting-edge, .Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols. is a valuable resource for researchers working in this important and developing field of study..
出版日期Book 2019
关键词Human induced pluripotent cells; Pharmaceuticals; Hepatocytes; Neuronal cells; Kidney cells; Cardiac cell
版次1
doihttps://doi.org/10.1007/978-1-4939-9477-9
isbn_softcover978-1-4939-9479-3
isbn_ebook978-1-4939-9477-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222929.jpg
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https://doi.org/10.1007/978-1-4939-9477-9Human induced pluripotent cells; Pharmaceuticals; Hepatocytes; Neuronal cells; Kidney cells; Cardiac cell
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978-1-4939-9479-3Springer Science+Business Media, LLC, part of Springer Nature 2019
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Improved Protocol for Cardiac Differentiation and Maturation of Pluripotent Stem Cellsle protocol for the differentiation of specific ventricular cardiomyocytes. These cells are elongated, do not spontaneously beat, and do not feature any Ca.-transient, an index of their stage of maturation toward adult cardiac cells. They represent a suitable model to screen both the efficiency and toxicology of drugs.
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,Ordering the Islands 1952–1958,l industry. The joint collaborative effort between academic and pharma partners within the StemBANCC consortium which enabled the implementation of iPSC-derived cellular models for drug discovery is highlighted. This large collaborative scientific network has successfully derived a significant numbe
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Oriental Identities in Super-Diverse Britaine physiologically relevant cell types in a robust and standardized way. Herein, we describe an efficient and scalable monolayer protocol to convert pluripotent stem cells into vascular endothelial cells using defined culture conditions..The combinatorial use of small molecule compounds, growth facto
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Oriental Identities in Super-Diverse Britainrce for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes in vitro. This protocol not only is chemically defined, that is, it does not use serum, but also allows for the expansion of astrocyte progenitor cells and mature astrocytes. Large batches o
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