Titlebook: Cell-Based Assays Using iPSCs for Drug Development and Testing; Carl-Fredrik Mandenius,James A. Ross Book 2019 Springer Science+Business M

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Differentiation of hiPS Cells into Definitive Endoderm for High-Throughput Screening of hiPS cells into DE cells is described. The produced DE cells were demonstrated to express classical DE markers on the gene expression and protein level. The here described DE cells are multipotent progenitors and act as starting points for a broad spectrum of endodermal model cells in HTS and other areas of drug discovery.

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Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiatoprotein in different multiwell formats. This provides a method to reliably produce more consistent hepatocytes in greater quantities and has enabled the development of high-throughput screening strategies.

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Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytesep sample preparation followed by 3.5-min analysis), as well as the possibility to localize possible enzyme deficiencies by quantifying the accumulation of specific intermediates involved in the synthetic pathways.

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Book 2019eagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Authoritative and cutting-edge, .Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols. is a valuable resource for researchers working in this important and developing field of study..

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Book 2019iology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endoth

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1064-3745 ation advice from the expertsThe volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human indu

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Oriental Identities in Super-Diverse Britainically defined, that is, it does not use serum, but also allows for the expansion of astrocyte progenitor cells and mature astrocytes. Large batches of hiPSc-derived astrocytes can be stored and used for defined in vitro disease models.