Titlebook: Cell Polarity Signaling; Methods and Protocol Chenbei Chang,Jianbo Wang Book 2022 Springer Science+Business Media, LLC, part of Springer Na

有角

Live-Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalics, endocytosis, exocytosis, and cytoskeletal reorganization. It is desirable to visualize these events without interference from other regions deeper within the cell. Total internal reflection fluorescence (TIRF) microscopy utilizes an elegant optical sectioning approach to visualize fluorophores n

Tailor

Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryoni what complexes are present in a cell, but information about the diversity of individual protein complexes is lost. Here, we describe single-cell, single-molecule pull-down (sc-SiMPull), a TIRF microscopy-based coimmunoprecipitation method, to visualize thousands of individual proteins, their bindin

Antagonist

Biochemical Assays to Detect Activation of Small GTPases Rho, Rac , and Cdc42 during Morphogenesis,the small GTPases Rho, Rac, and Cdc42, is one key mechanism that regulates cell polarity and migration during vertebrate gastrulation. In this chapter, we describe biochemical assays for detection of Wnt/Fz-mediated activation of Rho, Rac and Cdc42 in both mammalian cells and . embryo explants.

合并

名字

Regulation of Cell Polarity by Posttranslational Protein Palmitoylation, diseases. Therefore, the signaling pathways involved in establishing and maintaining cell polarity are tightly controlled. Protein S-palmitoylation has been recently recognized as an important posttranslational modification involved in cell polarity, via dynamic covalent attachment of fatty acyl gr

COWER

Enrichment and Detection of Wnt Proteins from Cell Culture Media, signaling range are under tight cellular control such that detection of Wnt proteins in biological samples is often extremely difficult. In this chapter, we describe a protocol to detect secreted Wnt proteins in the culture medium of cell lines that ectopically or endogenously express Wnt genes. Th

半球

Using Live Imaging to Examine Early Cardiac Development in Zebrafish,y. Similarly, live imaging can be vital to elucidating the pathology of genetic disorders and diseases. Model systems such as zebrafish, whose in vivo development is accessible to both the microscope and genetic manipulation, are particularly well-suited to the use of live imaging. Here we describe