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Titlebook: Cell Polarity Signaling; Methods and Protocol Chenbei Chang,Jianbo Wang Book 2022 Springer Science+Business Media, LLC, part of Springer Na

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书目名称Cell Polarity Signaling
副标题Methods and Protocol
编辑Chenbei Chang,Jianbo Wang
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell Polarity Signaling; Methods and Protocol Chenbei Chang,Jianbo Wang Book 2022 Springer Science+Business Media, LLC, part of Springer Na
描述This volume explores detailed methods to investigate various aspects of biology related to cell polarity, or asymmetry within a cell. Molecular, cellular, and tissue-level regulation and function as well as diseases caused by impairment of cell polarity are explored by these methods. Beginning with advanced imaging and biochemical methods, the book continues with planar cell polarity (PCP) signaling in morphogenesis in diverse developmental contexts, apicobasal (AB) cell polarity in development and diseases, as well as directional cell migration and biomechanics in cell polarity. The collection includes the usage of a wide variety of model systems and an extensive array of techniques, including genetic, imaging, biochemical, and biomechanical. Written for the highly successful .Methods in Molecular Biology. format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and comprehensive, .Cell Polarity Signaling: Methods and Protocols. aims to enable researchers to delve into the stimulating field of cell
出版日期Book 2022
关键词Directional migrating cells; Apicobasal polarity; Planar cell polarity; Cell asymmetry; Cultured cell sy
版次1
doihttps://doi.org/10.1007/978-1-0716-2035-9
isbn_softcover978-1-0716-2037-3
isbn_ebook978-1-0716-2035-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
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Erzeugung und Messung von Licht,n of interest. This method for detecting Wnt proteins will aid in the isolation of biologically active Wnt proteins, provide an assay to study the molecular basis of Wnt secretion, and potentially offer a means to detect trace amounts of Wnt proteins associated with pathological states.
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La forza della domanda di aiuto,t development. Whole-embryo culture and electroporation methods are also presented, allowing for pharmacological and genetic perturbation of SHF development, as well as image analysis approaches to quantify cell features across the progenitor cell epithelium. These protocols are broadly applicable to the study of epithelia in the early embryo.
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Live-Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internali for visualizing and tracking dynamics near the plasma membrane. In this chapter, we present a general experimental and analysis TIRF pipeline for studying cell surface receptor endocytosis. The approach presented can be easily applied to study other dynamic biological processes at or near the plasma membrane using TIRF microscopy.
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Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embrt development. Whole-embryo culture and electroporation methods are also presented, allowing for pharmacological and genetic perturbation of SHF development, as well as image analysis approaches to quantify cell features across the progenitor cell epithelium. These protocols are broadly applicable to the study of epithelia in the early embryo.
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