Titlebook: Cell Migration in Three Dimensions; Coert Margadant Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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https://doi.org/10.1007/b137561y encoded reporters of exosome secretion, pHluorin_M153R-CD63 and pHluorin_M153R-CD63-mScarlet. Here, we describe how to visualize secretion and uptake of exosomes labeled with these pH-sensitive and pH-insensitive fluorescent protein-tagged exosomal markers during cell migration using time-lapse fluorescent microscopy.

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Cell Migration in Three Dimensions978-1-0716-2887-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Obesity Management in Family Practicesion, and is therefore a widely studied phenomenon in many different fields from basic cell biology to regenerative medicine. During the past decades, a multitude of increasingly complex methods have been developed to study cell migration. Here we compile a series of current state-of-the-art methods

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Childhood and Adolescent Obesity, migration was previously understudied due to the limitations of 2D migration assays but has emerged as an important mode of migration in the past decade. Furthermore, confinement improves the quality of the imaging and simplifies the analysis of trajectories by confining migration to the plane of a