Titlebook: Cell Migration in Three Dimensions; Coert Margadant Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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书目名称Cell Migration in Three Dimensions网络公开度学科排名




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书目名称Cell Migration in Three Dimensions被引频次学科排名




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书目名称Cell Migration in Three Dimensions年度引用学科排名




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书目名称Cell Migration in Three Dimensions读者反馈学科排名

针叶树

Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroidsade, and processed to image mRNAs with single-molecule sensitivity. An analysis algorithm is used to quantify and compare mRNA distributions at the front of invasive leader cells. The approach can be easily adapted and applied to analyze RNA distributions in additional settings where cells polarize along a linear axis.

死亡

jeopardize

Obesity Management in Family Practiceating filopodia maps to compare the localization of multiple proteins within filopodia. Notably, while FiloMap was written to analyze filopodia, the analysis pipeline described here can also analyze and compile any line intensity profiles.

认识

Childhood and Adolescent Obesity,tact guidance studies. Then, we describe a method to perform the confinement on cells embedded inside a μm-thin 3D collagen gel. Finally, we describe an alternative method to confine cells based on agarose, so that cells can be fixed or drug perfused while being confined, which is currently not possible in the 2D confinement silicone-based device.

kindred

Mapping the Localization of Proteins Within Filopodia Using FiloMapating filopodia maps to compare the localization of multiple proteins within filopodia. Notably, while FiloMap was written to analyze filopodia, the analysis pipeline described here can also analyze and compile any line intensity profiles.

kindred

整体

1064-3745 expertsThis detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of param

Hormones

ALOFT

Visualization of Exosome Release and Uptake During Cell Migration Using the Live Imaging Reporter pHy encoded reporters of exosome secretion, pHluorin_M153R-CD63 and pHluorin_M153R-CD63-mScarlet. Here, we describe how to visualize secretion and uptake of exosomes labeled with these pH-sensitive and pH-insensitive fluorescent protein-tagged exosomal markers during cell migration using time-lapse fluorescent microscopy.