Titlebook: Cell Cycle Oscillators; Methods and Protocol Amanda S. Coutts,Louise Weston Book 2021Latest edition Springer Science+Business Media, LLC, p

Indebted

https://doi.org/10.1007/978-1-0716-1538-6Cellular interactions; Oscillatory networks; Developmental biology; Interlinking oscillators; Cell cycle

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1064-3745 expertsThis book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both

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Visualization of Radiation-Induced Cell Cycle Kinetics with a Fluorescent Ubiquitination-Based Cell G2 arrest in tumor cells with deficient p53 function. Here we describe protocols for establishing Fucci-expressing cell lines and analyzing radiation-induced G2 arrest kinetics in three different models: monolayer cell cultures, spheroids, and xenografted solid tumors in mice.

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,Construction of a Full-Length 3′UTR Reporter System for Identification of Cell-Cycle Regulating Mice to altered positioning of binding sites in respect to 3′UTR length and RNA secondary structure. Here we present a detailed protocol for the construction of full-length 3′UTR luciferase reporter constructs that was used to unveil miRNAs regulating multiple cell-cycle factors.

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Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells,d assay that enables the determination of cell cycle progression in the presence of CRISPR/Cas9 treatment, in addition to the transfection and expression efficiencies of Cas9 vectors. This assay can also easily determine the effect of various interventions on obtaining a larger pool of Cas9-treated cells.