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Titlebook: Cell Cycle Oscillators; Methods and Protocol Amanda S. Coutts,Louise Weston Book 2021Latest edition Springer Science+Business Media, LLC, p

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书目名称Cell Cycle Oscillators
副标题Methods and Protocol
编辑Amanda S. Coutts,Louise Weston
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell Cycle Oscillators; Methods and Protocol Amanda S. Coutts,Louise Weston Book 2021Latest edition Springer Science+Business Media, LLC, p
描述This book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives. The edition provides a space for researchers to highlight and explore the latest developments in molecular biology and biochemical techniques for studying oscillatory networks and to share these across the research community to facilitate further progress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cell Cycle Oscillators: Methods and Protocols, Second Edition serves as an invaluable guide for those new to the field as well as the more experienced scientist..Chapters 19 and 21 are available open access under a Creative Commons Attribution 4.0 International License via link.sprin
出版日期Book 2021Latest edition
关键词Cellular interactions; Oscillatory networks; Developmental biology; Interlinking oscillators; Cell cycle
版次2
doihttps://doi.org/10.1007/978-1-0716-1538-6
isbn_softcover978-1-0716-1540-9
isbn_ebook978-1-0716-1538-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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https://doi.org/10.1007/978-94-6209-622-6llators comprising of protein kinases and their regulators. Different cell cycle oscillators are inextricably linked to ensure orderly activation of oscillators. A recurring theme in their regulation is the abundance of autoamplifying loops that ensure switch-like and unidirectional cell cycle trans
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Origins: A Sustainable Concept in Educationn-dependent kinases (Cdks) and other cell cycle kinases. Here, I present the rationale and the experimental framework for an alternative approach to measure kinase activity that is based on estimating substrate phosphorylation rates in vivo. The approach presented was first developed for experiments
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Fred Dervin,Hanna Ragnarsdóttirthe APC/C targets its two essential substrates, securin and cyclin B, for proteasomal destruction. Cyclin B is the activator of cyclin-dependent kinase 1 (Cdk1), the major mitotic kinase, and both cyclin B and securin are safeguards of sister chromatid cohesion. Conversely, the degradation of securi
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https://doi.org/10.1007/978-94-6209-854-1ission. A wide variety of genetically-encoded and synthetic biosensors have been developed to monitor protein kinase activity. We have focused on the design, engineering and characterization of fluorescent peptide biosensors of cyclin-dependent kinases (CDKs) that constitute attractive cancer biomar
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On the Facilitation of the Academypression of mRNA expression. However, full-length 3′UTR vectors are rarely employed due to labor-intensive cloning work. Instead, 3′UTR fragments containing putative miRNA binding sites are commonly utilized to mechanistically validate miRNAs. Assaying truncated 3′UTRs may falsely validate miRNAs du
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