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Titlebook: Cell Culture Techniques in Heart and Vessel Research; H. M. Piper Book 1990 Springer-Verlag Berlin Heidelberg 1990 Endothelzellen.Kardiomy

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书目名称Cell Culture Techniques in Heart and Vessel Research
编辑H. M. Piper
视频video
图书封面Titlebook: Cell Culture Techniques in Heart and Vessel Research;  H. M. Piper Book 1990 Springer-Verlag Berlin Heidelberg 1990 Endothelzellen.Kardiomy
描述In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.
出版日期Book 1990
关键词Endothelzellen; Kardiomyozyten; Myokardiozyten; Myokardzell-Kultur; cardiovascular; cells; endothelium; gla
版次1
doihttps://doi.org/10.1007/978-3-642-75262-9
isbn_softcover978-3-642-75264-3
isbn_ebook978-3-642-75262-9
copyrightSpringer-Verlag Berlin Heidelberg 1990
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A. R. Mundy,J. M. Restorick,M. Bewickembryo heart fragments into single cells using trypsin was a significant technical advancement over past procedures, which included culture of whole salamander hearts, fetal mouse hearts, and fragments of chick heart. This method of isolating heart cells later became one of the common choices for ce
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https://doi.org/10.1007/978-94-011-6462-7ed away from embryonic chick heart isolated tissue expiants. Burrows suggested that his finding supported the myogenic theory for cardiac beating activity. Several decades passed before the realization that isolated systems, such as cells in culture, might serve aas useful tools for studying biologi
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Reasonable Attribution of Liabilitynates. A study of in vitro growth of an adult animal appeared only in 1974 [4]. In several attempts, isolated cardiomyocytes from adult animals either failed to establish in culture [5–8] or produced cells lacking convincing myotypic properties [9]. Most of the published work up to now has been cent
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The Nature of Occupational Health Disorders,ved from prenatal material obtained at a variety of gestational times. At first cultures were prepared from explanted tissue; 4- to 14-week embryos [36], 8-to 12-week embryos [29], 6- to 8-week embryos [19], a 13-week embryo [11], and 7- to 10-week embryos [5]. Development of methods for isolation o
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https://doi.org/10.1007/978-94-011-6893-9M) are critical to the regulation of the phenotype of the cell, which in turn is intimately associated with the particular function(s) of the cell. Changes in the chemical and physical composition of the ECM can cause altered cellular function as evidenced during the development of an organism or in
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https://doi.org/10.1007/978-94-011-6893-9ecent years. The culturing of striated cardiac myocytes even from the neonatal heart is hampered by a low biomass yield because of the limits of cell division [8] in cells derived from a tissue which is already amitotic 17 to 21 days postnatally [14]. Cultured heart cells have many advantages over n
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High-Power Semiconductor Laserslls. For a long time endothelial cells were thought to be rather inert cells forming a physical barrier on the inside of blood vessels. There was very little appreciation of the complex metabolic activity that these cells carry out. It is now clear that in addition to actively participating in the r
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