Titlebook: Caveolae; Methods and Protocol Cedric M. Blouin Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 electron micro

rectum

fetter

才能

金丝雀

,Investigation of Novel Cavin-1/Suppressor of Cytokine Signaling 3 (SOCS3) Interactions by Coimmunopon with a discrete motif within the SOCS3 SH2 domain. Here, we describe in detail three methods (coimmunoprecipitation; peptide pull-down; peptide array overlay) we have used to validate and characterize cavin-1/SOCS3 interactions in vitro.

显而易见

https://doi.org/10.1007/978-3-8349-4216-6ian cells with a CAV1-encoding plasmid or small interfering RNA (siRNA), analysis of mammalian cells by immunofluorescence microscopy (IFM) with antibodies against ciliary markers and CAV1, as well as methods for analyzing ciliary CAV1 function in siRNA-treated cells by IFM and cell-based signaling assays.

antidote

使成核

BUMP

Discrete Customer Choice Analysis membrane tethers, one can also probe the membrane reservoir of a cell and a sudden rise in the tether force is usually due to the depletion of excess membranes stored in membrane folds or invaginations.

MOT

Choice Situation in Low-Cost Marketsysiological setting and is thus suited for caveola research where observations within the tissues of an intact organism are increasingly relevant. This chapter also aims to introduce fundamental methodologies for the use of zebrafish in “in vivo cell biology.”

Coeval

Operationalizing Dynamic Pricing Models stress, and dyes such as calcein-AM and propidium iodide. We used this protocol for the in vitro study of the effect of mechanical stress on membrane integrity in human muscle cells from patients bearing caveolin-3 mutations.