书目名称 | Caveolae |
副标题 | Methods and Protocol |
编辑 | Cedric M. Blouin |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Caveolae: Methods and Protocols. is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and protein |
出版日期 | Book 2020 |
关键词 | electron microscopy; protein purification; fluorescence microscopy; spinning disk microscopy; endothelia |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-0716-0732-9 |
isbn_softcover | 978-1-0716-0734-3 |
isbn_ebook | 978-1-0716-0732-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |