Titlebook: Caveolae; Methods and Protocol Cedric M. Blouin Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 electron micro

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Caveolae978-1-0716-0732-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Overall Discussion and Conclusion,, transmission electron microscopy (TEM) has been the method of choice to study caveolae formation and ultrastructure and, more recently, to resolve the sub-caveolar localization of its protein components using novel protein labeling methods for TEM. This chapter describes a protocol for the selecti

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https://doi.org/10.1007/978-3-8349-4216-6 cell surface, and that Caveolin-1 (CAV1) plays important roles in regulating ciliary membrane composition and function. Here we describe methods to analyze the localization and function of CAV1 in primary cilia of cultured mammalian cells. These include methods for culturing and transfecting mammal

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Organisatorischer Wandel und Lernen,a membrane. Total internal reflection fluorescence microscopy exclusively illuminates molecules in the close vicinity of the glass surface, thereby reducing background fluorescence and enabling observation of the plasma membrane in the glass-attached cells with a high signal-to-noise ratio. Here, we

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https://doi.org/10.1007/978-3-8349-4485-6mple of a noninvasive technique that can be used to achieve this goal at nanometer resolution. FRET-based assays are dependent on the presence of fluorescent probes, such as CFP- and YFP-conjugated protein pairs. Here, we describe an experimental protocol in which live-cell FRET was used to measure